2022/04/12 Posters

【Poster_MBSJ 2016】Improvement of translational efficiency by N-terminal codon optimization in the reconstituted cell-free protein synthesis system

Keywords PUREfrex 2.0 / DsbC Set / DnaK Mix / N-terminal codon optimization / membrane protein synthesis / nanodisc / Fab antibody / PD-1 / PD-L1 /...

2022/04/12 Posters

【Poster_MBSJ 2015】 In vitro production of Fab, Toxin, and Immunotoxin using PUREfrex® 2.0 with increased productivity

Keywords PUREfrex® 2.0 / DsbC Set / DnaK Mix / scFv / Fab antibody / protein toxin / Gelonin / PE38 / antibody toxin conjugate / Immunotoxin Abstra...

2022/04/12 Posters

【Poster_MBSJ 2011】Synthesis of functionally active proteins containing disulfide bonds using the new PURE system (PUREfrex™)

Keywords PUREfrex® 1.0 / DsbC Set / disulfide bond formation / tissue plasminogen activator (tPA)/ acid phosphatase (AppA)/ alkaline phosphatase (A...

2020/12/21 Tech Notes

Sequence of DHFR DNA

DHFR DNA in the PUREfrex® kit is a PCR product containing a gene encoding dihydrofolate reductase (DHFR) from E.coli (18 kDa). The text shown belo...