2023/07/11 Tech Notes

Purification of His-tagged protein (DHFR-6xHis)

Since all of proteins involved in protein synthesis in PUREfrex® reaction mixture are free of His-tag, the protein of interest can be synth...

2021/02/04 Tech Notes

6 Tips about the template DNA Design

Certain nucleotide or amino acid sequence may reduce the yield of the protein of interest. In such cases, optimizing the sequence may improve the y...

2020/12/22 Tech Notes

Preparation of the template DNA

The design and preparation of template DNA for PUREfrex® is a very important step for successful protein synthesis. The following is a summary of t...

2020/12/21 Tech Notes

Sequence of DHFR DNA

DHFR DNA in the PUREfrex® kit is a PCR product containing a gene encoding dihydrofolate reductase (DHFR) from E.coli (18 kDa). The text shown belo...