2021/02/09 Tech Notes

Synthesis of Protein Toxin (Gelonin) using DnaK Mix

Gelonin is a 30 kDa potein toxin from plant Gelonium multiflorum. It is know as one of the ribosome-inactivating protein (RIP) and has rRNA N-glyco...

2021/02/04 Tech Notes

6 Tips about the template DNA Design

Certain nucleotide or amino acid sequence may reduce the yield of the protein of interest. In such cases, optimizing the sequence may improve the y...

2020/12/22 Tech Notes

Preparation of the template DNA

The design and preparation of template DNA for PUREfrex® is a very important step for successful protein synthesis. The following is a summary of t...

2020/12/21 Tech Notes

Sequence of DHFR DNA

DHFR DNA in the PUREfrex® kit is a PCR product containing a gene encoding dihydrofolate reductase (DHFR) from E.coli (18 kDa). The text shown belo...