Synthesis of Protein Toxin (Gelonin) using DnaK Mix
Gelonin is a 30 kDa potein toxin from plant Gelonium multiflorum. It is know as one of the ribosome-inactivating protein (RIP) and has rRNA N-glyco...
6 Tips about the template DNA Design
Certain nucleotide or amino acid sequence may reduce the yield of the protein of interest. In such cases, optimizing the sequence may improve the y...
Preparation of the template DNA
The design and preparation of template DNA for PUREfrex® is a very important step for successful protein synthesis. The following is a summary of t...
Sequence of DHFR DNA
DHFR DNA in the PUREfrex® kit is a PCR product containing a gene encoding dihydrofolate reductase (DHFR) from E.coli (18 kDa). The text shown belo...