Unique cell-free protein synthesis system, PUREfrex – Useful platform for protein expression in the development of biologics -
Abstruct PUREfrex® is a cell-free synthesis system based on PURE (Protein synthesis Using Recombinant Element) system. PURE system is a reconstitut...
Boundary-Free Ribosome Compartmentalization by Gene Expression on a Surface
Michael Levy, Reuven Falkovich, Ohad Vonshak, Dan Bracha, Alexandra M Tayar, Yoshihiro Shimizu, Shirley S Daubev and Roy H Bar-Ziv. ACS Synth Biol....
Synthesis of Protein Toxin (Gelonin) using DnaK Mix
Gelonin is a 30 kDa potein toxin from plant Gelonium multiflorum. It is know as one of the ribosome-inactivating protein (RIP) and has rRNA N-glyco...
Cell-Free Expression of a Plant Membrane Protein BrPT2 From Boesenbergia Rotunda
Yvonne Jing Mei Liew, Yean Kee Lee, Norzulaani Khalid, Noorsaadah Abd Rahman and Boon Chin Tan. Mol Biotechnol. 2021 Apr;63(4):316-326. https://doi...
6 Tips about the template DNA Design
Certain nucleotide or amino acid sequence may reduce the yield of the protein of interest. In such cases, optimizing the sequence may improve the y...
Search for translation arrest peptides encoded upstream of genes for components of protein localization pathways
Karen Sakiyama, Naomi Shimokawa-Chiba, Keigo Fujiwara and Shinobu Chiba. Nucleic Acids Res. 2021 Feb 22;49(3):1550-1566. https://doi.org/10.1093/na...