【Poster_MBSJ 2011】Synthesis of functionally active proteins containing disulfide bonds using the new PURE system (PUREfrex™)
Keywords PUREfrex® 1.0 / DsbC Set / disulfide bond formation / tissue plasminogen activator (tPA)/ acid phosphatase (AppA)/ alkaline phosphatase (A...
6 Tips about the template DNA Design
Certain nucleotide or amino acid sequence may reduce the yield of the protein of interest. In such cases, optimizing the sequence may improve the y...
Preparation of the template DNA
The design and preparation of template DNA for PUREfrex® is a very important step for successful protein synthesis. The following is a summary of t...
Sequence of DHFR DNA
DHFR DNA in the PUREfrex® kit is a PCR product containing a gene encoding dihydrofolate reductase (DHFR) from E.coli (18 kDa). The text shown belo...