Day 3

Rebuilding the Cell-Free System and Their Applications for R&D of Biologics

Wednesday, May 4  10 am

Downloadable slides.


What is PUREfrex®

Rebuilding the Cell-Free System

PUREfrex® is a reconstituted cell-free protein synthesis kit which GeneFrontier has developed based on the PURE system technology. It consists of only purified factors necessary for transcription, translation and energy regeneration. The target protein is synthesized by adding the template DNA (or mRNA) to the reaction mixture. The synthesized protein with a tag can be easily purified because all of proteins in the PUREfrex® contain no tags.

Our products

Cell-free Protein Synthesis Kits

  • PUREfrex® 2.0   - for Protein Synthesis -
  • PUREfrex® 2.1  - for Forming Disulfide Bonds -
  • PUREfrex® 1.0  - for Basic Research and Customized kit -

Supplements & Other proteins

  • DnaK Mix  – Chaperones, HSP70 protein –
  • GroE Mix  – Chaperones, HSP60 protein –
  • DsbC Mix  – Forming disulfide bonds, E.coli disulfide isomerase –
  • PDI Mix  – Forming disulfide bonds, Human disulfide isomerase –
  • EF-P  - translation factor -