Regulated N-Terminal Modification of Proteins Synthesized Using a Reconstituted Cell-Free Protein Synthesis System.

[Publication year] 2023
[Journal] ACS Synth. Biol. 12, 1935–1942
[Authors]  Rena Matsumoto, Tatsuya Niwa, Yasuhiro Shimane, Yutetsu Kuruma, Hideki Taguchi, and Takashi Kanamori
https://pubs.acs.org/doi/full/10.1021/acssynbio.3c00191

 

In vitro reconstitution of the Escherichia coli 70S ribosome with a full set of recombinant ribosomal proteins.

[Publication year] 2022
[Journal] J. Biochem. 171, 227-237.
[Authors]  Ryo Aoyama, Keiko Masuda, Masaru Shimojo, Takashi Kanamori, Takuya Ueda and Yoshihiro Shimizu.
https://academic.oup.com/jb/article/171/2/227/6423477

 

Identification of conditions for efficient cell-sized liposome preparation using commercially available reconstituted in vitro transcription-translation system.

[Publication year] 2022
[Journal] J Biosci Bioeng. 133, 181-186.
[Authors]  Atsuko Uyeda, Sabrina Galiñanes Reyes, Takashi Kanamori and Tomoaki Matsuura.
https://www.sciencedirect.com/science/article/abs/pii/S1389172321002905

 

In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli.

[Publication year] 2020
[Journal] Commun. Biol. 3, 142.
[Authors]  Shimojo M, Amikura K, Masuda K, Kanamori T, Ueda T and Shimizu Y.
https://www.nature.com/articles/s42003-020-0874-8

 

Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system.

[Publication year] 2019
[Journal] Scientific reports. 9, 671.
[Authors]  Satoshi Murakami, Rena Matsumoto,  Takashi Kanamori
https://www.nature.com/articles/s41598-018-36691-8

 

Development of human neutralizing antibody to ADAMTS4 (aggrecanase-1) and ADAMTS5 (aggrecanase-2).

[Publication year] 2016
[Journal] Biochem. Biophys. Res. Commun. 469, 62-69.
[Authors]  Shiraishi, A., Mochizuki, S., Miyakoshi, A., Kojoh, K., & Okada, Y.
https://www.sciencedirect.com/science/article/abs/pii/S0006291X15309414

 

PURE ribosome display and its application in antibody technology.

[Publication year] 2014
[Journal] Biochim Biophys Acta. 1844, 1925-1932.
[Authors]  Kanamori T, Fujino Y and Ueda T.
https://www.sciencedirect.com/science/article/abs/pii/S1570963914000934

 

Protein synthesis yield increased 72 times in the cell-free PURE system.

[Publication year] 2014
[Journal] Integrative Biology. 6, 781-788.
[Authors]  Jackson, K., Kanamori, T., Ueda, T., & Fan, Z. H.
https://academic.oup.com/ib/article-abstract/6/8/781/5199011

 

The PURE System for Protein Production.

[Publication year] 2013
[Journal] Methods Mol. Biol. 1118, 275-284.
[Authors]  Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, and Takuya Ueda
https://link.springer.com/protocol/10.1007/978-1-62703-782-2_19

 

Global analysis of chaperone effects using a reconstituted cell-free translation system.

[Publication year] 2012
[Journal] Proc Natl Acad Sci U S A. 109, 8937-8942.
[Authors]  Niwa T, Kanamori T, Ueda T, Taguchi H.
https://www.pnas.org/doi/abs/10.1073/pnas.1201380109

 

Elongation factor G is a critical target during oxidative damage to the translation system of Escherichia coli.

[Publication year] 2012
[Journal] J. Biol. Chem. 287, 28697-28704.
[Authors]  Takanori Nagano, Kouji Kojima, Toru Hisabori, Hidenori Hayashi, Eugene Hayato Morita, Takashi Kanamori, Tomoko Miyagi, Takuya Ueda, and Yoshitaka Nishiyama
https://www.jbc.org/article/S0021-9258(20)68405-8/fulltext

 

Robust in vitro affinity maturation strategy based on interface-focused high-throughput mutational scanning.

[Publication year] 2012
[Journal] Biochem. Biophys. Res. Commun. 428, 395-400.
[Authors]  Yasuhiro Fujino, Risako Fujita, Kouichi Wada, Kotomi Fujishige, Takashi Kanamori, Lindsey Hunt, Yoshihiro Shimizu, and Takuya Ueda
https://www.sciencedirect.com/science/article/abs/pii/S0006291X12020384

 

Peptide screening using PURE ribosome display.

[Publication year] 2011
[Journal] Methods Mol. Biol. 805, 251-259.
[Authors]  Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada, Bintang K Akbar, and Takuya Ueda
https://link.springer.com/protocol/10.1007/978-1-61779-379-0_14

 

Recruitment of a species-specific translational arrest module to monitor different cellular processes.

[Publication year] 2011
[Journal] Proc. Natl. Acad. Sci. U. S. A. 108, 6073-6078.
[Authors]  Shinobu Chiba, Takashi Kanamori, Takuya Ueda, Yoshinori Akiyama, Kit Pogliano, and Koreaki Ito
https://www.pnas.org/doi/abs/10.1073/pnas.1018343108

 

A novel complete reconstitution system for membrane integration of the simplest membrane protein.

[Publication year] 2010
[Journal] Biochem. Biophys. Res. Commun. 394, 733-736.
[Authors]  Ken-Ichi Nishiyama, Masahide Maeda, Masato Abe, Takashi Kanamori, Keiko Shimamoto, Shoichi Kusumoto, Takuya Ueda, and Hajime Tokuda
https://www.sciencedirect.com/science/article/abs/pii/S0006291X10005127

 

Ribosome display with the PURE technology.

[Publication year] 2009
[Journal] Methods Mol. Biol. 607, 219-225.
[Authors]  Takuya Ueda, Takashi Kanamori, and Hiroyuki Ohashi
https://link.springer.com/protocol/10.1007/978-1-60327-331-2_18

 

Epitope mapping using ribosome display in a reconstituted cell-free protein synthesis system.

[Publication year] 2009
[Journal] J Biochem. 145, 693-700.
[Authors]  Osada E, Shimizu Y, Akbar BK, Kanamori T, Ueda T.
https://academic.oup.com/jb/article-abstract/145/5/693/777052

 

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