Our papers with PUREfrex®
Effect of Translation-Enhancing Nascent SKIK Peptide on the Arrest Peptides Containing Consecutive Proline
[Publication year] 2024
[Journal] ACS Synth Biol. 2024 Nov 21. Online ahead of print.
[Authors] Nishikawa Y, Fujikawa R, Nakano H, Kanamori T, and Ojima-Kato T.
https://pubs.acs.org/doi/10.1021/acssynbio.4c00221
N-Terminal Amino Acid Affects the Translation Efficiency at Lower Temperatures in a Reconstituted Protein Synthesis System.
[Publication year] 2024
[Journal] Int. J. Mol. Sci. 25, 5264
[Authors] Tomoe Fuse-Murakami, Rena Matsumoto, and Takashi Kanamori
https://www.mdpi.com/1422-0067/25/10/5264
Mechanistic dissection of premature translation termination induced by acidic residues-enriched nascent peptide.
[Publication year] 2023
[Journal] Cell Rep. 42, 113569.
[Authors] Chadani Y, Kanamori T, Niwa T, Ichihara K, Nakayama KI, Matsumoto A, and Taguchi H.
https://www.cell.com/cell-reports/fulltext/S2211-1247(23)01581-4
Regulated N-Terminal Modification of Proteins Synthesized Using a Reconstituted Cell-Free Protein Synthesis System.
[Publication year] 2023
[Journal] ACS Synth. Biol. 12, 1935–1942
[Authors] Rena Matsumoto, Tatsuya Niwa, Yasuhiro Shimane, Yutetsu Kuruma, Hideki Taguchi, and Takashi Kanamori
https://pubs.acs.org/doi/full/10.1021/acssynbio.3c00191
In vitro reconstitution of the Escherichia coli 70S ribosome with a full set of recombinant ribosomal proteins.
[Publication year] 2022
[Journal] J. Biochem. 171, 227-237.
[Authors] Ryo Aoyama, Keiko Masuda, Masaru Shimojo, Takashi Kanamori, Takuya Ueda and Yoshihiro Shimizu.
https://academic.oup.com/jb/article/171/2/227/6423477
Identification of conditions for efficient cell-sized liposome preparation using commercially available reconstituted in vitro transcription-translation system.
[Publication year] 2022
[Journal] J Biosci Bioeng. 133, 181-186.
[Authors] Atsuko Uyeda, Sabrina Galiñanes Reyes, Takashi Kanamori and Tomoaki Matsuura.
https://www.sciencedirect.com/science/article/abs/pii/S1389172321002905
In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli.
[Publication year] 2020
[Journal] Commun. Biol. 3, 142.
[Authors] Shimojo M, Amikura K, Masuda K, Kanamori T, Ueda T and Shimizu Y.
https://www.nature.com/articles/s42003-020-0874-8
Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system.
[Publication year] 2019
[Journal] Scientific reports. 9, 671.
[Authors] Satoshi Murakami, Rena Matsumoto, Takashi Kanamori
https://www.nature.com/articles/s41598-018-36691-8
Development of human neutralizing antibody to ADAMTS4 (aggrecanase-1) and ADAMTS5 (aggrecanase-2).
[Publication year] 2016
[Journal] Biochem. Biophys. Res. Commun. 469, 62-69.
[Authors] Shiraishi, A., Mochizuki, S., Miyakoshi, A., Kojoh, K., & Okada, Y.
https://www.sciencedirect.com/science/article/abs/pii/S0006291X15309414
PURE ribosome display and its application in antibody technology.
[Publication year] 2014
[Journal] Biochim Biophys Acta. 1844, 1925-1932.
[Authors] Kanamori T, Fujino Y and Ueda T.
https://www.sciencedirect.com/science/article/abs/pii/S1570963914000934
Protein synthesis yield increased 72 times in the cell-free PURE system.
[Publication year] 2014
[Journal] Integrative Biology. 6, 781-788.
[Authors] Jackson, K., Kanamori, T., Ueda, T., & Fan, Z. H.
https://academic.oup.com/ib/article-abstract/6/8/781/5199011
The PURE System for Protein Production.
[Publication year] 2013
[Journal] Methods Mol. Biol. 1118, 275-284.
[Authors] Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, and Takuya Ueda
https://link.springer.com/protocol/10.1007/978-1-62703-782-2_19
Global analysis of chaperone effects using a reconstituted cell-free translation system.
[Publication year] 2012
[Journal] Proc Natl Acad Sci U S A. 109, 8937-8942.
[Authors] Niwa T, Kanamori T, Ueda T, Taguchi H.
https://www.pnas.org/doi/abs/10.1073/pnas.1201380109
Elongation factor G is a critical target during oxidative damage to the translation system of Escherichia coli.
[Publication year] 2012
[Journal] J. Biol. Chem. 287, 28697-28704.
[Authors] Takanori Nagano, Kouji Kojima, Toru Hisabori, Hidenori Hayashi, Eugene Hayato Morita, Takashi Kanamori, Tomoko Miyagi, Takuya Ueda, and Yoshitaka Nishiyama
https://www.jbc.org/article/S0021-9258(20)68405-8/fulltext
Robust in vitro affinity maturation strategy based on interface-focused high-throughput mutational scanning.
[Publication year] 2012
[Journal] Biochem. Biophys. Res. Commun. 428, 395-400.
[Authors] Yasuhiro Fujino, Risako Fujita, Kouichi Wada, Kotomi Fujishige, Takashi Kanamori, Lindsey Hunt, Yoshihiro Shimizu, and Takuya Ueda
https://www.sciencedirect.com/science/article/abs/pii/S0006291X12020384
Peptide screening using PURE ribosome display.
[Publication year] 2011
[Journal] Methods Mol. Biol. 805, 251-259.
[Authors] Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada, Bintang K Akbar, and Takuya Ueda
https://link.springer.com/protocol/10.1007/978-1-61779-379-0_14
Recruitment of a species-specific translational arrest module to monitor different cellular processes.
[Publication year] 2011
[Journal] Proc. Natl. Acad. Sci. U. S. A. 108, 6073-6078.
[Authors] Shinobu Chiba, Takashi Kanamori, Takuya Ueda, Yoshinori Akiyama, Kit Pogliano, and Koreaki Ito
https://www.pnas.org/doi/abs/10.1073/pnas.1018343108
A novel complete reconstitution system for membrane integration of the simplest membrane protein.
[Publication year] 2010
[Journal] Biochem. Biophys. Res. Commun. 394, 733-736.
[Authors] Ken-Ichi Nishiyama, Masahide Maeda, Masato Abe, Takashi Kanamori, Keiko Shimamoto, Shoichi Kusumoto, Takuya Ueda, and Hajime Tokuda
https://www.sciencedirect.com/science/article/abs/pii/S0006291X10005127
Ribosome display with the PURE technology.
[Publication year] 2009
[Journal] Methods Mol. Biol. 607, 219-225.
[Authors] Takuya Ueda, Takashi Kanamori, and Hiroyuki Ohashi
https://link.springer.com/protocol/10.1007/978-1-60327-331-2_18
Epitope mapping using ribosome display in a reconstituted cell-free protein synthesis system.
[Publication year] 2009
[Journal] J Biochem. 145, 693-700.
[Authors] Osada E, Shimizu Y, Akbar BK, Kanamori T, Ueda T.
https://academic.oup.com/jb/article-abstract/145/5/693/777052