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Tech Notes
6 Tips about the template DNA Design
Certain nucleotide or amino acid sequence may reduce the yield of the protein of interest. In such cases, optimizing the sequence may improve the y...
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Tech Notes
Preparation of the template DNA
The design and preparation of template DNA for PUREfrex® is a very important step for successful protein synthesis. The following is a summary of t...
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Tech Notes
Purification of His-tagged protein (DHFR-6xHis)
Since all of proteins involved in protein synthesis in PUREfrex® reaction mixture are free of His-tag, the protein of interest can be synth...