Lunch Workshop
Wednesday, 31 May 12:15 PM - 1:15 PM
Rebuilt Cell-Free System (PUREfrex®); From basic research to industrial application.
Abstract: Our unique platform technology named PUREfrex® is a fully reconstituted (or rebuilt) cell-free protein expression system. It's easy to customize the system for various applications at molecular basis. It's been widely used not only for the expression of difficult-to-express proteins but also for the platform of synthetic biology. We'll give a flavor of huge potential of our technology in this field from basic research to industrial application.
Speaker: Takashi Ebihara, Ph.D. COO, GeneFrontier Corporation
Downloadable slides.
Stop by our booth to meet our latest Rebuilding the Cell Free System “PUREfrex®”.
What is PUREfrex®
Rebuilding the Cell-Free System
PUREfrex® is a reconstituted cell-free protein synthesis kit which GeneFrontier has developed based on the PURE (Protein synthesis Using Recombinant Elements) system technology. It consists of only purified factors necessary for transcription, translation and energy regeneration. The target protein is synthesized by adding the template DNA (or mRNA) to the reaction mixture. The synthesized protein with a tag can be easily purified because all of proteins in the PUREfrex® contain no tags.
Our products
Cell-free Protein Synthesis Kits
- PUREfrex® 2.0 - for Protein Synthesis -
- PUREfrex® 2.1 - for Forming Disulfide Bonds -
- PUREfrex® 1.0 - for Basic Research and Customized kit -
Supplements & Other proteins
- DnaK Mix – Chaperones, HSP70 protein –
- GroE Mix – Chaperones, HSP60 protein –
- DsbC Mix – Forming disulfide bonds, E.coli disulfide isomerase –
- PDI Mix – Forming disulfide bonds, Human disulfide isomerase –
- EF-P - translation factor -