2023/07/11 Tech Notes

Purification of His-tagged protein (DHFR-6xHis)

Since all of proteins involved in protein synthesis in PUREfrex® reaction mixture are free of His-tag, the protein of interest can be synth...

2023/06/28 Cases

N-terminal modification of cell-free synthesized proteins (acetylation and myristoylation).

The N-terminal modification of nascent proteins, such as acetylation and myristoylation, is one of the most abundant post-translational modificatio...

2022/04/12 Posters

【Poster_MBSJ 2021】 Synthesis of glycosylated proteins using PUREfrex® with glycosyltransferase

Keywords PUREfrex® 2.1 / DnaK Mix / in vitro glycosylation (IVG) / glycosyltransferase / N-linked glycoprotein / one-pot cell-free protein synthesi...

2022/04/12 Posters

【Poster_MBSJ 2021】Exploration of the template DNA sequence suitable for the E. coli-based reconstituted cell-free protein synthesis system (PUREfrex®)

Keywords PUREfrex® 2.1 / Template DNA optimization / 5′UTR optimization / Open reading frame (ORF) optimization Abstract PUREfrex® is a build up ce...

2022/04/12 Posters

【Poster_PSSJ 2021】Protein synthesis form the bicistronic template DNA using the E.coli-based reconstituted cell-free protein synthesis system (PUREfrex®)

Keywords PUREfrex® 2.0 / bicistronic template DNA / 5′UTR / AT-rich region / ribosome binding sequence / mCherry / GFP Abstract PUREfrex® is a buil...

2022/04/12 Posters

【Poster_MBSJ 2020】Investigation on how to synthesize active proteins by using a reconstituted cell-free protein synthesis system (PUREfrex®)

Keywords PUREfrex® 2.1 / DnaK Mix / PDI Set / optimization of synthesis conditions / protein solubilization / surfactant / molecular chaperone / Cl...

2022/04/12 Posters

【Poster_MBSJ 2018】Improvement of the translation efficiency of proline residue-containing proteins using the reconstituted cell-free protein synthesis sytem (PUREfrex)

Keywords PUREfrex® 2.0 / EF-P / proteins containing consecutive prolines / Release Factor 2 (RF2) / AmiB / PfkB / IL-6 / Pro-GFP / DHFR-Pro Abstrac...

2022/04/12 Posters

【Poster_PSSJ 2018】Synthesis of proteins containing disulfide bonds using PUREfrex® with human protein disulfide isomerase

Keywords PUREfrex® 2.1 / PDI Set / DsbC Set / disulfide bond formation / human protein disulfide isomerase (hPDI) / human endoplasmic oxidereductin...

2022/04/12 Posters

【Poster_PSSJ 2017】 Analysis of the redox state in a reconstituted cell-free protein synthesis system (PUREfrex®)

Keywords PUREfrex® 2.0 / reaction mixture oxidation / redox state / cysteinylation / reducing reagent / disulfide bond formation Abstract PUREfrex®...

2022/04/12 Posters

【Poster_PSSJ 2017】Development of a method for the synthesis of aglycosylated full-length IgG using PUREfrex

Keywords PUREfrex® 2.1 / DsbC Set / DnaK Mix / cell-free IgG synthesis / Trastuzumab / IgG1 / IgG2 / IgG4 Abstract Aglycosylated IgG (including IgG...