Publication on N-terminal modification of cell-free synthesized proteins using the PUREfrex® has been released.
A paper on N-terminal modification of cell-free synthesized proteins using PUREfrex® has been published in ACS Synthetic Biology. This is a collaboration between Gene Frontier, Tokyo Institute of Technology, and the Japan Agency for Marine-Earth Science and Technology (JAMSTEC).
In this study, we developed a preparation method for the N-terminal modification of cell-free synthesized proteins using the PURE system (Product name: PUREfrex®). We also synthesized the modifying enzymes using the PURE system and used them without purification. This approach may be useful for evaluating the activity of modifying enzymes without using living cells. Our PURE system-based method can not only be applied to achieve acetylation and myristoylation, but also other modification.
Regulated N-Terminal Modification of Proteins Synthesized Using a Reconstituted Cell-Free Protein Synthesis System
ACS Synthetic Biology (2023) 12, 1935-1942.
Download paper: https://pubs.acs.org/doi/10.1021/acssynbio.3c00191
Summary article: [Cases] N-terminal modification of cell-free synthesized proteins (acetylation and myristoylation).